The Effects of Actin Stability on Neural Cell Biology
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biological and Environmental Sciences
Date of Award
Fall 2025
Abstract
Neural development is significantly influenced by cytoskeletal dynamics. Understanding cytoskeletal dynamics can provide insights into the mechanisms of neural development and function in health and pathology. This study aims to investigate the effects of actin stability and dynamics on neuron development. Actin stability or instability were established through pharmacological manipulations in early developmental stages of Drosophila melanogaster. Embryos were permeabilized and exposed to either Latrunculin A, an actin depolymerizing agent, or Jasplakinolide, an actin stabilizer. Following the manipulations, immunostaining assay was utilized to visualize the neuronal extensions in later developmental stages. Results of the study indicate that actin depolymerizing treatments during embryonic stage significantly reduced bouton formation and active zone measured in larval stage, suggesting compromised synaptic connectivity. On the other hand, actin stabilizing treatments during embryonic stage caused excessive bouton formation measured in larval stage suggesting potential disruption of neural integrity. Altogether, these results highlight the important role of actin dynamics in neuromuscular junction formation and offer more insights into the mechanism underlying motor neuron function.
Advisor
Hunkar Gizem Yesilyurt
Subject Categories
Life Sciences
Recommended Citation
Le, Vi, "The Effects of Actin Stability on Neural Cell Biology" (2025). Electronic Theses & Dissertations. 1327.
https://lair.etamu.edu/etd/1327
